The MMSF houses two Thermo LCQdeca-MS, a Thermo LCQdeca XPplus MS, a Micromass Quattro Ultima Triple Quadrupole MS, an Agilent 6230 Accurate-Mass TOFMS, an Agilent 5977B GC-MSD, and a Bruker Biflex IV MALDI-TOFMS. Details on each instrument are shown as followed:
The Thermo LCQdeca is an advanced analytical instrument that includes a syringe pump, a divert/inject valve, an atmospheric pressure ionization source (electrospray ionization, ESI, or atmospheric pressure chemical ionization source, APCI), a quadrupole ion trap mass analyzer, and the Xcalibur data system. The instrument is equipped with a HP 1100 LC station. Pure samples can be analyzed by flow injection using the inject valve fitted with a loop or direct infusion. Mixtures can be separated into its various components by a LC column and then be analyzed by a diode-array UV detector and the MS detector. The MS detector can be operated under both positive and negative ion modes with the capabilities of doing MS/MS and MSn analysis. The instrument has unit mass resolution over a mass range of 50-2000 m/z with a typical mass accuracy of 100 ppm. Typical sample types suitable for analysis on the LCQdeca-MS include synthetic organic molecules, synthetic peptides, natural products, organometallic compounds, lipids, and intact proteins.
The Thermo LCQdeca XPplus couples an orthogonal API source (ESI or APCI) with a Quadrupole Ion Trap Mass Analyzer. With enhanced ion optics and Ion SweepTM gas, the LCQdeca XPplus delivers superior sensitivity and performance for a variety of advanced LC-MSn applications, making it one of the benchmark LC-MSn instruments for applications such as metabolism and proteomics. Fully automated MSn capabilities and advanced Data DependentTM experiment design features make analysis of low-abundance compounds in any matrix routine. Typical sample types suitable for analysis on the LCQdeca XPplus MS include synthetic organic molecules, synthetic peptides, natural products, organometallic compounds, lipids, and intact proteins.
The Micromass Quattro Ultima is a high performance benchtop triple quadrupole mass spectrometer designed for routine LC-MS and LC-MS/MS operations. It may be configured with any of the following three options: an HPLC system with an autosampler; an infusion pump; or a a syringe pump. Ionization takes place in the source at atmospheric pressure. These ions are sampled through a series of orifices into the first quadrupole where they are filtered according to their mass to charge ratio (m/z). The mass separated ions then pass into the hexapole collision cell where they either undergo collision induced decomposition (CID) or pass unhindered to the second quadrupole. The fragment ions are then mass analyzed by the second quadrupole. Finally the transmitted ions are detected by a conversion dynode, phosphor and photomultiplier detection system. The output signal is amplified, digitised and presented to the data system. The Quattro Ultima is especially useful in drug metabolism and pharmacokinetics (DMPK) study, and the analysis of synthetic organic molecules, synthetic peptides, natural products, lipids, and intact proteins. Contact Dr. Su if you want training on the Micromass Triple Quadrupole MS.
The Agilent 6230 Accurate-Mass TOFMS system features Agilent Jet Stream Thermal Focusing technology for significantly improved sensitivity, as well as enhanced MassHunter Workstation software for superior data mining and analysis capabilities. These new features, coupled with Agilent’s True High-Definition TOF technology, enable the 6230 Accurate-Mass TOF to deliver exceptional sensitivity, excellent mass accuracy, fast data acquisition, and streamlined qualitative and quantitative analysis. An Agilent 1260 Infinity Binary LC is coupled with this 6230 Accurate-Mass TOFMS system for on-line HPLC-HR-ESI/APCI-MS analysis. Typical sample types suitable for analysis on the Agilent 6230 LC-TOFMS include synthetic organic molecules, synthetic peptides, natural products, organometallic compounds, and proteins.
The Agilent 5977B Gas Chromatograph-Mass Selective Detector (GC-MSD) instrument builds on a tradition of leadership in GC and MS technology, with excellent best performance and productivity features. This robust instrument has electron impact sources coupled with an Agilent 7820A GC system. Equipped with an Agilent 7693 Autosampler, it can be used for rapid, high-throughput mass analyses with high detection sensitivity and mass accuracy. The Agilent 7820A GC unit makes the analysis of mixtures (e.g., to follow the progress of a reaction) possible. The instrument is particularly useful for routine analysis of non-polar small organic molecules in the mass range of 50-1000 amu. The instrument is available to trained users 7/24. Contact Dr. Su if you want training on the Agilent 5977B GC-MSD.
Matrix-assisted laser desorption Ionization (MALDI) coupled with time of flight (TOF) mass analyzer is a powerful tool for characterization of biopolymers (proteins, protein digests and oligonucleotides), organometallic complexes, synthetic polymers, and other high molecular weight organic materials. The Bruker Biflex IV MALDI-TOFMS uses 337nm laser for desorption and ionization, and can be operated under linear mode (125 cm effective flight path) or reflectron mode (270 cm effective flight path) with outstanding analytical performance. The Bruker Biflex IV MALDI-TOFMS can provide a typical mass resolution of >4,000 (FWHM, at m/z=2465.2, i.e. ACTH 18-39) for linear mode and >20,000 (FWHM, at m/z=3147.5, i.e. Somatostatin 28) for reflectron mode), a typical mass accuracy of <100 ppm for linear mode and <10 ppm for reflectron mode, and a mass range up to ~300,000 Da. The instrument is available to trained users 7/24. Contact Dr. Su if you want training on the Bruker Biflex IV MALDI-TOFMS.